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paper n a popink votu  (Addgene inc)


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    Addgene inc paper n a popink votu
    Paper N A Popink Votu, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+n+a+popink+votu/pOPINK-vOTU+(CCHFV+OTU%2C+aa+1-183)+(Plasmid+%2361589)/pm41167191-489-242-251
    Average 93 stars, based on 6 article reviews
    paper n a popink votu - by Bioz Stars, 2026-09
    93/100 stars

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    Mutagenesis:

    Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GeneArtTM Site-Directed Mutagenesis System Thermo Fisher Scientific Cat# A13282 RNeasy plus Mini kit Qiagen Cat# 74134 SuperScriptTM II Reverse Transcriptase Thermo Fisher Scientific Cat# 18064014 GoTaq® qPCR Master Mix Promega Cat# A6001 Deposited data Raw mass spectrometry proteomics data ProteomeXchange PRIDE: PXD066536 Raw RNA-seq data NCBI SRA: PRJNA1295885 Unprocessed and uncompressed data This paper, Mendeley data Mendeley Data: https://doi.org/ 10.17632/t2bdkr74s7.1 Experimental Models: Cell Lines Human U2OS ATCC Cat# HTB-96; RRID: CVCL_0042 Human HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human HCT116 ATCC Cat# CCL-247; RRID: CVCL_0291 Human SW480 ATCC Cat# CCL-228; RRID: CVCL_0546 L-WRN ATCC Cat# CRL-3276; RRID: CVCL_DA06 Experimental models: Organisms/strains C57BL/6J Rnf32-/- mice This paper N/A Oligonucleotides ON-TARGETplus SMARTpool RNF32 siRNA oligonucleotides Dharmacon Cat# L-007136-00-0005 Recombinant DNA pCMV3-C-FLAG-RNF32 Sino Biological Cat# HG24274-UT pET-21a-hCaM GenScript N/A pcDNA3.1-hCaM-TEV-MYC-HIS This paper N/A pcDNA3.1-FLAG-FLAG-HA-HA-RNF32 WT and mutants This paper N/A pcDNA3.1-HA-RNF32 This paper N/A pEGFP-RNF32 This paper N/A pCMV-TAG-NEMO Wu et al. 28 Addgene Cat# 11970 pmCherry-NEMO This paper N/A pEF4-FLAG-IKKα Provided by V. D’Angiolella N/A pEF4-FLAG-IKKβ Provided by V. D’Angiolella N/A pNf-ĸB-luc Provided by S. Papa N/A pRL-TK Provided by S. Papa N/A pcDNA3.1-His-Ubiquitin-WT This paper N/A pcDNA3.1-His-Ubiquitin-K/R mutants This paper N/A pWC7-His-MYC-Ubiquitin WT and mutants This paper N/A pcDNA3.1-FLAG-Ubiquitin-K63-only This paper N/A pHAGE2-EF1α-IRES-puromycin-RNF32 WT and mutants This paper N/A pcDNA3.1-FLAG-STREP-RNF32 This paper N/A pET-3a-Ubiquitin This paper N/A pET21-His-UBA1 This paper N/A pET15-His-UbE2D2 This paper N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al. 29 Addgene Cat# 48138 pGEX-USP2 (aa 259-605) This paper N/A pOPINK-vOTU (aa 1-183) Akutsu et al. 30 Addgene Cat# 61589 pProEx-hOTUB1 Wang et al. 31 Addgene Cat# 26959 (Continued on next page) Molecular Cell 85, 1–14.e1–e9, November 20, 2025 e3

    Reverse Transcription:

    Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GeneArtTM Site-Directed Mutagenesis System Thermo Fisher Scientific Cat# A13282 RNeasy plus Mini kit Qiagen Cat# 74134 SuperScriptTM II Reverse Transcriptase Thermo Fisher Scientific Cat# 18064014 GoTaq® qPCR Master Mix Promega Cat# A6001 Deposited data Raw mass spectrometry proteomics data ProteomeXchange PRIDE: PXD066536 Raw RNA-seq data NCBI SRA: PRJNA1295885 Unprocessed and uncompressed data This paper, Mendeley data Mendeley Data: https://doi.org/ 10.17632/t2bdkr74s7.1 Experimental Models: Cell Lines Human U2OS ATCC Cat# HTB-96; RRID: CVCL_0042 Human HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human HCT116 ATCC Cat# CCL-247; RRID: CVCL_0291 Human SW480 ATCC Cat# CCL-228; RRID: CVCL_0546 L-WRN ATCC Cat# CRL-3276; RRID: CVCL_DA06 Experimental models: Organisms/strains C57BL/6J Rnf32-/- mice This paper N/A Oligonucleotides ON-TARGETplus SMARTpool RNF32 siRNA oligonucleotides Dharmacon Cat# L-007136-00-0005 Recombinant DNA pCMV3-C-FLAG-RNF32 Sino Biological Cat# HG24274-UT pET-21a-hCaM GenScript N/A pcDNA3.1-hCaM-TEV-MYC-HIS This paper N/A pcDNA3.1-FLAG-FLAG-HA-HA-RNF32 WT and mutants This paper N/A pcDNA3.1-HA-RNF32 This paper N/A pEGFP-RNF32 This paper N/A pCMV-TAG-NEMO Wu et al. 28 Addgene Cat# 11970 pmCherry-NEMO This paper N/A pEF4-FLAG-IKKα Provided by V. D’Angiolella N/A pEF4-FLAG-IKKβ Provided by V. D’Angiolella N/A pNf-ĸB-luc Provided by S. Papa N/A pRL-TK Provided by S. Papa N/A pcDNA3.1-His-Ubiquitin-WT This paper N/A pcDNA3.1-His-Ubiquitin-K/R mutants This paper N/A pWC7-His-MYC-Ubiquitin WT and mutants This paper N/A pcDNA3.1-FLAG-Ubiquitin-K63-only This paper N/A pHAGE2-EF1α-IRES-puromycin-RNF32 WT and mutants This paper N/A pcDNA3.1-FLAG-STREP-RNF32 This paper N/A pET-3a-Ubiquitin This paper N/A pET21-His-UBA1 This paper N/A pET15-His-UbE2D2 This paper N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al. 29 Addgene Cat# 48138 pGEX-USP2 (aa 259-605) This paper N/A pOPINK-vOTU (aa 1-183) Akutsu et al. 30 Addgene Cat# 61589 pProEx-hOTUB1 Wang et al. 31 Addgene Cat# 26959 (Continued on next page) Molecular Cell 85, 1–14.e1–e9, November 20, 2025 e3

    Real-time Polymerase Chain Reaction:

    Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GeneArtTM Site-Directed Mutagenesis System Thermo Fisher Scientific Cat# A13282 RNeasy plus Mini kit Qiagen Cat# 74134 SuperScriptTM II Reverse Transcriptase Thermo Fisher Scientific Cat# 18064014 GoTaq® qPCR Master Mix Promega Cat# A6001 Deposited data Raw mass spectrometry proteomics data ProteomeXchange PRIDE: PXD066536 Raw RNA-seq data NCBI SRA: PRJNA1295885 Unprocessed and uncompressed data This paper, Mendeley data Mendeley Data: https://doi.org/ 10.17632/t2bdkr74s7.1 Experimental Models: Cell Lines Human U2OS ATCC Cat# HTB-96; RRID: CVCL_0042 Human HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human HCT116 ATCC Cat# CCL-247; RRID: CVCL_0291 Human SW480 ATCC Cat# CCL-228; RRID: CVCL_0546 L-WRN ATCC Cat# CRL-3276; RRID: CVCL_DA06 Experimental models: Organisms/strains C57BL/6J Rnf32-/- mice This paper N/A Oligonucleotides ON-TARGETplus SMARTpool RNF32 siRNA oligonucleotides Dharmacon Cat# L-007136-00-0005 Recombinant DNA pCMV3-C-FLAG-RNF32 Sino Biological Cat# HG24274-UT pET-21a-hCaM GenScript N/A pcDNA3.1-hCaM-TEV-MYC-HIS This paper N/A pcDNA3.1-FLAG-FLAG-HA-HA-RNF32 WT and mutants This paper N/A pcDNA3.1-HA-RNF32 This paper N/A pEGFP-RNF32 This paper N/A pCMV-TAG-NEMO Wu et al. 28 Addgene Cat# 11970 pmCherry-NEMO This paper N/A pEF4-FLAG-IKKα Provided by V. D’Angiolella N/A pEF4-FLAG-IKKβ Provided by V. D’Angiolella N/A pNf-ĸB-luc Provided by S. Papa N/A pRL-TK Provided by S. Papa N/A pcDNA3.1-His-Ubiquitin-WT This paper N/A pcDNA3.1-His-Ubiquitin-K/R mutants This paper N/A pWC7-His-MYC-Ubiquitin WT and mutants This paper N/A pcDNA3.1-FLAG-Ubiquitin-K63-only This paper N/A pHAGE2-EF1α-IRES-puromycin-RNF32 WT and mutants This paper N/A pcDNA3.1-FLAG-STREP-RNF32 This paper N/A pET-3a-Ubiquitin This paper N/A pET21-His-UBA1 This paper N/A pET15-His-UbE2D2 This paper N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al. 29 Addgene Cat# 48138 pGEX-USP2 (aa 259-605) This paper N/A pOPINK-vOTU (aa 1-183) Akutsu et al. 30 Addgene Cat# 61589 pProEx-hOTUB1 Wang et al. 31 Addgene Cat# 26959 (Continued on next page) Molecular Cell 85, 1–14.e1–e9, November 20, 2025 e3

    Mass Spectrometry:

    Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GeneArtTM Site-Directed Mutagenesis System Thermo Fisher Scientific Cat# A13282 RNeasy plus Mini kit Qiagen Cat# 74134 SuperScriptTM II Reverse Transcriptase Thermo Fisher Scientific Cat# 18064014 GoTaq® qPCR Master Mix Promega Cat# A6001 Deposited data Raw mass spectrometry proteomics data ProteomeXchange PRIDE: PXD066536 Raw RNA-seq data NCBI SRA: PRJNA1295885 Unprocessed and uncompressed data This paper, Mendeley data Mendeley Data: https://doi.org/ 10.17632/t2bdkr74s7.1 Experimental Models: Cell Lines Human U2OS ATCC Cat# HTB-96; RRID: CVCL_0042 Human HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human HCT116 ATCC Cat# CCL-247; RRID: CVCL_0291 Human SW480 ATCC Cat# CCL-228; RRID: CVCL_0546 L-WRN ATCC Cat# CRL-3276; RRID: CVCL_DA06 Experimental models: Organisms/strains C57BL/6J Rnf32-/- mice This paper N/A Oligonucleotides ON-TARGETplus SMARTpool RNF32 siRNA oligonucleotides Dharmacon Cat# L-007136-00-0005 Recombinant DNA pCMV3-C-FLAG-RNF32 Sino Biological Cat# HG24274-UT pET-21a-hCaM GenScript N/A pcDNA3.1-hCaM-TEV-MYC-HIS This paper N/A pcDNA3.1-FLAG-FLAG-HA-HA-RNF32 WT and mutants This paper N/A pcDNA3.1-HA-RNF32 This paper N/A pEGFP-RNF32 This paper N/A pCMV-TAG-NEMO Wu et al. 28 Addgene Cat# 11970 pmCherry-NEMO This paper N/A pEF4-FLAG-IKKα Provided by V. D’Angiolella N/A pEF4-FLAG-IKKβ Provided by V. D’Angiolella N/A pNf-ĸB-luc Provided by S. Papa N/A pRL-TK Provided by S. Papa N/A pcDNA3.1-His-Ubiquitin-WT This paper N/A pcDNA3.1-His-Ubiquitin-K/R mutants This paper N/A pWC7-His-MYC-Ubiquitin WT and mutants This paper N/A pcDNA3.1-FLAG-Ubiquitin-K63-only This paper N/A pHAGE2-EF1α-IRES-puromycin-RNF32 WT and mutants This paper N/A pcDNA3.1-FLAG-STREP-RNF32 This paper N/A pET-3a-Ubiquitin This paper N/A pET21-His-UBA1 This paper N/A pET15-His-UbE2D2 This paper N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al. 29 Addgene Cat# 48138 pGEX-USP2 (aa 259-605) This paper N/A pOPINK-vOTU (aa 1-183) Akutsu et al. 30 Addgene Cat# 61589 pProEx-hOTUB1 Wang et al. 31 Addgene Cat# 26959 (Continued on next page) Molecular Cell 85, 1–14.e1–e9, November 20, 2025 e3

    RNA Sequencing:

    Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GeneArtTM Site-Directed Mutagenesis System Thermo Fisher Scientific Cat# A13282 RNeasy plus Mini kit Qiagen Cat# 74134 SuperScriptTM II Reverse Transcriptase Thermo Fisher Scientific Cat# 18064014 GoTaq® qPCR Master Mix Promega Cat# A6001 Deposited data Raw mass spectrometry proteomics data ProteomeXchange PRIDE: PXD066536 Raw RNA-seq data NCBI SRA: PRJNA1295885 Unprocessed and uncompressed data This paper, Mendeley data Mendeley Data: https://doi.org/ 10.17632/t2bdkr74s7.1 Experimental Models: Cell Lines Human U2OS ATCC Cat# HTB-96; RRID: CVCL_0042 Human HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human HCT116 ATCC Cat# CCL-247; RRID: CVCL_0291 Human SW480 ATCC Cat# CCL-228; RRID: CVCL_0546 L-WRN ATCC Cat# CRL-3276; RRID: CVCL_DA06 Experimental models: Organisms/strains C57BL/6J Rnf32-/- mice This paper N/A Oligonucleotides ON-TARGETplus SMARTpool RNF32 siRNA oligonucleotides Dharmacon Cat# L-007136-00-0005 Recombinant DNA pCMV3-C-FLAG-RNF32 Sino Biological Cat# HG24274-UT pET-21a-hCaM GenScript N/A pcDNA3.1-hCaM-TEV-MYC-HIS This paper N/A pcDNA3.1-FLAG-FLAG-HA-HA-RNF32 WT and mutants This paper N/A pcDNA3.1-HA-RNF32 This paper N/A pEGFP-RNF32 This paper N/A pCMV-TAG-NEMO Wu et al. 28 Addgene Cat# 11970 pmCherry-NEMO This paper N/A pEF4-FLAG-IKKα Provided by V. D’Angiolella N/A pEF4-FLAG-IKKβ Provided by V. D’Angiolella N/A pNf-ĸB-luc Provided by S. Papa N/A pRL-TK Provided by S. Papa N/A pcDNA3.1-His-Ubiquitin-WT This paper N/A pcDNA3.1-His-Ubiquitin-K/R mutants This paper N/A pWC7-His-MYC-Ubiquitin WT and mutants This paper N/A pcDNA3.1-FLAG-Ubiquitin-K63-only This paper N/A pHAGE2-EF1α-IRES-puromycin-RNF32 WT and mutants This paper N/A pcDNA3.1-FLAG-STREP-RNF32 This paper N/A pET-3a-Ubiquitin This paper N/A pET21-His-UBA1 This paper N/A pET15-His-UbE2D2 This paper N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al. 29 Addgene Cat# 48138 pGEX-USP2 (aa 259-605) This paper N/A pOPINK-vOTU (aa 1-183) Akutsu et al. 30 Addgene Cat# 61589 pProEx-hOTUB1 Wang et al. 31 Addgene Cat# 26959 (Continued on next page) Molecular Cell 85, 1–14.e1–e9, November 20, 2025 e3

    Recombinant:

    Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GeneArtTM Site-Directed Mutagenesis System Thermo Fisher Scientific Cat# A13282 RNeasy plus Mini kit Qiagen Cat# 74134 SuperScriptTM II Reverse Transcriptase Thermo Fisher Scientific Cat# 18064014 GoTaq® qPCR Master Mix Promega Cat# A6001 Deposited data Raw mass spectrometry proteomics data ProteomeXchange PRIDE: PXD066536 Raw RNA-seq data NCBI SRA: PRJNA1295885 Unprocessed and uncompressed data This paper, Mendeley data Mendeley Data: https://doi.org/ 10.17632/t2bdkr74s7.1 Experimental Models: Cell Lines Human U2OS ATCC Cat# HTB-96; RRID: CVCL_0042 Human HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human HCT116 ATCC Cat# CCL-247; RRID: CVCL_0291 Human SW480 ATCC Cat# CCL-228; RRID: CVCL_0546 L-WRN ATCC Cat# CRL-3276; RRID: CVCL_DA06 Experimental models: Organisms/strains C57BL/6J Rnf32-/- mice This paper N/A Oligonucleotides ON-TARGETplus SMARTpool RNF32 siRNA oligonucleotides Dharmacon Cat# L-007136-00-0005 Recombinant DNA pCMV3-C-FLAG-RNF32 Sino Biological Cat# HG24274-UT pET-21a-hCaM GenScript N/A pcDNA3.1-hCaM-TEV-MYC-HIS This paper N/A pcDNA3.1-FLAG-FLAG-HA-HA-RNF32 WT and mutants This paper N/A pcDNA3.1-HA-RNF32 This paper N/A pEGFP-RNF32 This paper N/A pCMV-TAG-NEMO Wu et al. 28 Addgene Cat# 11970 pmCherry-NEMO This paper N/A pEF4-FLAG-IKKα Provided by V. D’Angiolella N/A pEF4-FLAG-IKKβ Provided by V. D’Angiolella N/A pNf-ĸB-luc Provided by S. Papa N/A pRL-TK Provided by S. Papa N/A pcDNA3.1-His-Ubiquitin-WT This paper N/A pcDNA3.1-His-Ubiquitin-K/R mutants This paper N/A pWC7-His-MYC-Ubiquitin WT and mutants This paper N/A pcDNA3.1-FLAG-Ubiquitin-K63-only This paper N/A pHAGE2-EF1α-IRES-puromycin-RNF32 WT and mutants This paper N/A pcDNA3.1-FLAG-STREP-RNF32 This paper N/A pET-3a-Ubiquitin This paper N/A pET21-His-UBA1 This paper N/A pET15-His-UbE2D2 This paper N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al. 29 Addgene Cat# 48138 pGEX-USP2 (aa 259-605) This paper N/A pOPINK-vOTU (aa 1-183) Akutsu et al. 30 Addgene Cat# 61589 pProEx-hOTUB1 Wang et al. 31 Addgene Cat# 26959 (Continued on next page) Molecular Cell 85, 1–14.e1–e9, November 20, 2025 e3



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    paper n a popink votu  (Addgene inc)
    93
    Addgene inc paper n a popink votu
    Paper N A Popink Votu, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+n+a+popink+votu/pOPINK-vOTU+(CCHFV+OTU%2C+aa+1-183)+(Plasmid+%2361589)/pm41167191-489-242-251
    Average 93 stars, based on 1 article reviews
    paper n a popink votu - by Bioz Stars, 2026-09
    93/100 stars
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